Pollen is collected on the fur of bee when it is foraging for nectar. The bee gently moves this pollen into little pollen sacks on it's back legs. This pollen is used to feed the young of the hive and also for pollination. When the bee returns to the hive, some of this pollen is knocked off when it enters the hive. This is what we collect. There is no detrimental effect to the sustainability of the hive.